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CRISPR/Cas9 Mutagenesis in Phlebotomus papatasi: the Immune Deficiency Pathway Impacts Vector Competence for Leishmania major

ABSTRACT Sand flies are the natural vectors for the Leishmania species that produce a spectrum of diseases in their mammalian hosts, including humans.Studies of sand fly/Leishmania interactions have been limited by the absence of genome editing techniques applicable to these insects.In this report, we adapted CRISPR (clustered regularly interspaced

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Determination of deoxycholic acid pool size and input rate using [24-13C]deoxycholic acid and serum sampling.

We have developed an isotope dilution method for determination of deoxycholic acid pool size and input rate which employs oral administration of 50 mg of [24-13C]deoxycholic acid and serum sampling.The HI POTENCY MULTI method has been validated by classical isotope dilution technique using [24-14C]deoxycholic acid and bile sampling in five patients

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